Picas L, Milhiet PE, Hernández-Borrell J. Chem Phys Lipids. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. A Thermodynamic Study of the Effects of Cholesterol on the Interaction between Liposomes and Ethanol. Magneto-Liposomes as MRI Contrast Agents: A Systematic Study of Different Liposomal Formulations. This site needs JavaScript to work properly. Kostevšek N, Cheung CCL, Serša I, Kreft ME, Monaco I, Comes Franchini M, Vidmar J, Al-Jamal WT. 2007;400:503-13. doi: 10.1007/978-1-59745-519-0_34. 2015 Jul 21;11(27):5447-54. doi: 10.1039/c5sm01252j. Biochemistry. In addition, even when no thermal transition is detected by the traditional bulk analysis techniques for liposomes with high Chol content (40 and 50 mol %), we demonstrate that temperature-controlled AFM-FS is capable of identifying a thermal transition for the supported lipid bilayers. We use cookies to help provide and enhance our service and tailor content and ads. Benesch MG, Mannock DA, Lewis RN, McElhaney RN.  |  Clipboard, Search History, and several other advanced features are temporarily unavailable. NLM It determines the fluidity and permeability of the membrane, making it permeable to water but not to ions and protons. Impact of galactosylceramides on the nanomechanical properties of lipid bilayer models: an AFM-force spectroscopy study. Epub 2017 Jul 3. Fernandes TFD, Saavedra-Villanueva O, Margeat E, Milhiet PE, Costa L. Sci Rep. 2020 Apr 27;10(1):7098. doi: 10.1038/s41598-020-62529-3. Cholesterol (Chol) plays the essential function of regulating the physical properties of the cell membrane by controlling the lipid organization and phase behavior and, thus, managing the membrane fluidity and its mechanical strength. The phase transition temperature is defined as the temperature required to induce a change in the lipid physical state from the ordered gel phase, where the hydrocarbon chains are fully extended and closely packed, to the disordered liquid crystalline phase, where … Copyright © 2000 The Biophysical Society. Bilayer cholesterol was varied from 20 mol% to 40 mol%. We prove that low Chol contents lead to a phase-segregated system, whereas high contents of Chol can give a homogeneous bilayer. https://doi.org/10.1016/S0006-3495(00)76793-2. Anticancer Ruthenium(III) Complexes and Ru(III)-Containing Nanoformulations: An Update on the Mechanism of Action and Biological Activity. By this means, we correlate the mechanical behavior and composition with the lateral order of each phase present in the bilayers. In particular, the existence of dynamically coexisting domains of gel and fluid lipids in the transition temperature region may play an important role for association of ethanol with the lipid bilayers. Soft Matter. In the low concentration regime (<10 mol%), cholesterol enhances the affinity of ethanol for the lipid bilayer compared to pure DMPC bilayers, whereas higher levels of cholesterol (>10 mol%) reduce affinity of ethanol for the lipid bilayer. Please enable it to take advantage of the complete set of features! 2011 Nov 22;50(46):9982-97. doi: 10.1021/bi200721j.  |  USA.gov. Increasing the bilayer cholesterol content above 20 mol% reduced that permeability peak. NIH Finally, our results validate the AFM-FS technique as an ideal platform to differentiate phase coexistence and transitions in lipid bilayers and bridge the gap between the results obtained by traditional methods for bulk analysis, the theoretical predictions, and the behavior of these systems at the nanoscale. doi: 10.7554/eLife.52535. Cholesterol gradually increases the temperature at which the L α to L o phase transition occurs. Get the latest public health information from CDC: https://www.coronavirus.gov. 2020 Mar 30;9:e52535. By continuing you agree to the use of cookies. Published by Elsevier Inc. All rights reserved. ACS Biomater Sci Eng. Tuberculosis-associated IFN-I induces Siglec-1 on tunneling nanotubes and favors HIV-1 spread in macrophages. 2015 Oct;191:123-35. doi: 10.1016/j.chemphyslip.2015.09.002. In both cases, Chol enhances the mechanical stability of the membrane, and an extraordinarily stable system is observed for equimolar fractions (50 mol % Chol). The calorimetric data show that the interaction of ethanol with the lipid membranes is endothermic and strongly dependent on the phase behavior of the mixed lipid bilayer, specifically whether the lipid bilayer is in the solid ordered (so), liquid disordered (ld), or liquid ordered (lo) phase. Therefore, under these conditions cholesterol increases the melting point. 2019 Sep 26;12(4):146. doi: 10.3390/ph12040146. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. As it appears from the phase diagram, only a marginal reduction in the transition temperature of the main phase transition occurs. Riccardi C, Musumeci D, Trifuoggi M, Irace C, Paduano L, Montesarchio D. Pharmaceuticals (Basel). Miscibility transition temp vs EtOH conc. Glucose permeation rates were measured from 10°C to 36°C, and showed a maximum in permeability at 24°C, the DMPC phase transition temperature. Atomic force microscopy: a versatile tool to probe the physical and chemical properties of supported membranes at the nanoscale. Chem Phys Lipids. Here, we explore the model system DPPC:Chol by means of temperature-controlled atomic force microscopy (AFM) imaging and AFM-based force spectroscopy (AFM-FS) to assess the influence of Chol on the membrane ordering and stability. Cholesterol also regulates the functions of the transporters and signaling proteins present on the plasma membrane.